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Estimation of the expression level of adipogenic markers of bone marrow-derived mesenchymal stem cells. Relative mRNA expression of (A) Ppar - γ , (B) C/EBP - α , (C) C/EBP - β , (D) C/EBP - γ , (E) Lpl , (F) ap2 , (G) Adipoq , and (H) Cfd as estimated from quantitative real-time reverse transcription-polymerase chain reaction. (I) Representative immunoblots of Ppar-γ, C/EBP-α, and ap2 indicate the expression pattern of the tested markers at the protein levels. Statistically significant differences were indicated. a) P <0.05, b) P <0.01, c) P <0.001 compared with undifferentiated control.

Journal: Journal of Bone Metabolism

Article Title: Comparison of Differences in Cell Migration during the Osteogenic and Adipogenic Differentiation of the Bone Marrow-Derived Stem Cells

doi: 10.11005/jbm.25.841

Figure Lengend Snippet: Estimation of the expression level of adipogenic markers of bone marrow-derived mesenchymal stem cells. Relative mRNA expression of (A) Ppar - γ , (B) C/EBP - α , (C) C/EBP - β , (D) C/EBP - γ , (E) Lpl , (F) ap2 , (G) Adipoq , and (H) Cfd as estimated from quantitative real-time reverse transcription-polymerase chain reaction. (I) Representative immunoblots of Ppar-γ, C/EBP-α, and ap2 indicate the expression pattern of the tested markers at the protein levels. Statistically significant differences were indicated. a) P <0.05, b) P <0.01, c) P <0.001 compared with undifferentiated control.

Article Snippet: Membranes were probed with the mentioned primary antibodies; Runx2 1:1,000 (Cat. No. 12556; Cell Signaling Technology, Danvers, MA, USA), Bmp2 1:1,000 (Cat. No. ab284387; Abcam, Cambridge, MA, USA), Col1a1 (Cat. No. A1352; ABclonal Science, Inc., Woburn, MA, USA), ap2 1:1,000 (Cat. No. 15872-1-AP; Proteintech, Chicago, USA), PPAR-γ 1:500 (Cat. No. ab19481; Abcam), C/EBP-α 1:500 (Cat. No. 74404; Abcam), Vcam1 1:2,000 (Cat. No. A19131PM; ABclonal), Cxcr4 1:1,000 (Cat. No. sc-53534; Santa Cruz Biotechnology, Dallas, TX, USA), Sdf-1 1:1,000 (Cat. No. A18225; ABclonal), Itgα5 1:1,000 (Cat. No. A19069; ABclonal), Itgα1 1:1,000 (Cat. No. A16054; ABclonal) or β-actin 1:50,000 (Cat. No. A3854; Sigma-Aldrich) overnight at 4°C.

Techniques: Expressing, Derivative Assay, Reverse Transcription, Polymerase Chain Reaction, Western Blot, Control